报告题目：Protein Interaction Inside Live Cells
主讲人：Mikael Oliveberg
时 间：2024年11月18日10:00
地 点：安徽大学磬苑校区文典阁
主办单位：生命科学学院
欢迎各位老师、同学届时前往！
摘要: A distinctive characteristic of nucleic acids and lipid membranes, as well as the majority of intracellular proteins, is their net negative charge. This negative charge is thought to play a critical role in maintaining basal intermolecular repulsion, ensuring a suitably fluid cytosolic environment for cellular function. This seminar explores the experimental, theoretical, and genetic evidence supporting this concept and examines the new questions it raises. Unlike controlled test-tube conditions, functional protein-protein interactions in the cytosol are constantly challenged by competition from densely crowded, sticky backgrounds. At the nonspecific end of this stickiness spectrum lies random protein-protein associations, which maintain vast populations of transient and continuously interconverting complexes at physiological concentrations. This phenomenon is effectively measured through studies of protein rotational diffusion, showing that increased net negative charge reduces clustering and facilitates protein dynamics. Additionally, this intricate protein interplay is evolutionarily fine-tuned across organisms to sustain optimal cellular physicochemical conditions. Emerging evidence suggests that specific cellular functions depend on the tight competition between numerous weak and strong interactions, with all regions of protein surfaces contributing. The outstanding challenge is to unravel the fundamental principles of this complex system: understanding how precise patterns of charged, polar, and hydrophobic side chains regulate both close- and long-range protein interactions, as well as the collective properties of the cellular interior.
报告人简介：Mikael Oliveberg教授是瑞典皇家科学院院士，诺贝尔奖评委，是国际著名的蛋白质生物物理学家。他曾担任或现任多个重要委员会和机构的领导职务，瑞典国家学校教育委员会委员、瑞典于默奥大学生物医学研究院主席和技术学院董事等。Oliveberg教授荣获过瑞典皇家科学院古斯塔夫森化学奖等国家级和国际大奖。每年，他受邀在国际会议上做五次以上的大会报告。近年来，他的研究主要集中在蛋白质折叠与淀粉样聚集、肌萎缩侧索硬化症（ALS）的发生机制以及蛋白质在细胞内的物理化学特性等方面，取得了众多重要成果。迄今为止，他已发表超过100篇SCI论文，其中30余篇以通讯作者身份在影响因子大于10的国际顶级期刊上发表。